Optimization of Heparin Monitoring with Anti-FXa Assays and the Impact of Dextran Sulfate for Measuring All Drug Activity

Heparins, unfractionated or low molecular weight, are permanently in the spotlight of both clinical indications and laboratory monitoring. An accurate drug dosage is necessary for an efficient safe therapy. The one-stage kinetic anti-FXa assays most widely universally used with full automation large series, without needing exogenous antithrombin. WHO International Standards available UFH LMWH, but external quality assessment surveys still report a high inter-assay variability. This heterogeneity results from following: assay formulation, designed dextran sulfate to measure all heparin blood circulation; calibrators testing LMWH same curve; parameters. In this study, various factors which impact measurements reviewed, we share our experience optimize anticoagulant activities plasma. Evidence provided on usefulness weight completely mobilize present circulation. Other key concern adjustment conditions obtain fully superimposable calibration curves calibrators’ formulations, illustrate performances different treated patients’ plasmas obtained using citrate CTAD anticoagulants. Comparable only when used. Using as anticoagulant, underestimated absence sulfate. Heparin calibrators, parameters, data treatment contribute other smaller differences.